Pseudomonas protegens

Rhizosphere Probiotic for Plant Resilience & Root Growth

Pseudomonas protegens is a fast-colonizing, non-spore-forming soil probiotic and plant growth-promoting rhizobacterium (PGPR) widely used as a biostimulant and biofertilizer to enhance plant nutrition, root development, and stress tolerance. This beneficial soil bacteria supports rhizosphere balance, solubilizes key nutrients like phosphorus and iron, and activates systemic signaling for improved crop performance. Suitable for regenerative and conventional agriculture, especially where growers aim to reduce synthetic inputs.

Primes plant immune signaling via ISR-associated pathways to support defense readiness under environmental and microbial stress
Reinforces rhizosphere health by promoting microbial diversity and producing biosurfactants that shape beneficial soil microbe interactions
Stimulates root elongation, branching, and seedling establishment through hormone-like microbial metabolites characteristic of PGPR
Increases nutrient bioavailability as a phosphate-solubilizing bacterium, producing siderophores and organic acids that mobilize iron, phosphorus, and trace elements
Supports plant metabolism and resilience under salinity, drought, and nutrient-deficient conditions with active microbial synergy

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Modulates Rhizosphere Microbial Balance

Enhances microbial diversity and stability in the root zone by fostering competitive and beneficial ecological interactions among soil fertility microbes.

Competes for space and nutrients to maintain a resilient rhizobacterial community
Produces metabolites such as 2,4-diacetylphloroglucinol that influence microbial dynamics and promote root-zone stability
Encourages colonization by beneficial microbes for plants, supporting long-term rhizosphere function

Supports Soil Microbial Ecology in Challenging Environments

Functions as a soil inoculant that improves conditions in soils prone to biological imbalance or abiotic stress through microbial action.

Produces biosurfactants and bioactive compounds that influence the composition of the rhizosphere
Stimulates enzymatic activity and organic matter transformation for nutrient cycling
Helps suppress microbial conditions associated with poor fertility through beneficial microbial interactions

Induces Systemic Resistance (ISR) in Plants

Activates plant defense signaling pathways that help crops better tolerate environmental and biological stress without functioning as a direct biocontrol agent.

Triggers jasmonic acid and ethylene pathways involved in systemic defense and stress mitigation
Enhances resilience in plants exposed to root-zone microbial pressure and abiotic extremes
Complements integrated crop programs by reducing reliance on chemical tools through natural biostimulant activity

Enhances Root Development and Plant Growth

Works as a microbial inoculant to stimulate vigorous root growth and seedling establishment through PGPR-mediated metabolite signaling and microbial support.

Produces auxin-like compounds that promote root elongation, branching, and nutrient foraging
Improves seedling uniformity, early vigor, and adaptability in variable soil conditions
Contributes to plant-microbe synergy at the root interface

Improves Nutrient Solubilization and Uptake

Facilitates the availability and assimilation of key macronutrients and micronutrients through metabolite-driven soil activity.

Acts as a phosphate-solubilizing and iron-chelating bacterium via organic acid and siderophore production
Enhances nitrogen uptake through synergy with other soil fertility microbes in the rhizosphere

Contributes to Soil Health and Organic Matter Cycling

Strengthens soil structure and biological activity through enzymatic transformation and microbial turnover.

Produces extracellular enzymes (e.g., cellulases, dehydrogenases) that break down organic residues
Boosts humus formation and soil aggregation for improved aeration and moisture retention
Serves as a growth-promoting bacteria that contributes to long-term soil quality in organic farming microbe programs

Improves Abiotic Stress Tolerance

Enhances plant performance under drought, salinity, and nutrient stress through microbial-plant interactions.

Increases antioxidant enzyme activity in host plants, supporting cellular stability
Helps maintain osmotic balance and nutrient flow in saline or low-moisture conditions
Supports adaptive growth and stress recovery through metabolite contributions typical of growth promoters.

Why Choose Our Pseudomonas protegens?

High CFU Powder for Rapid Colonization and Resilience - Our Pseudomonas protegens is formulated as a high-viability powdered microbial inoculant designed for fast colonization of the rhizosphere. Its aggressive root zone establishment ensures consistent results across diverse soil types and cropping systems.
Activates Systemic Resistance Without Biocidal Action - Stimulates induced systemic resistance (ISR) pathways, enhancing plant readiness against environmental and microbial stressors without acting as a pesticide. This PGPR-mediated signaling supports crop resilience under drought, salinity, and biological pressure.
Promotes Microbial Balance and Rhizosphere Diversity - Reinforces beneficial microbial communities through biosurfactant production and competitive colonization, helping to stabilize soil ecosystems. Ideal for maintaining rhizosphere equilibrium in stressed or degraded soils.
Solubilizes Nutrients Through Organic Acids and Siderophores - Functions as a phosphate-solubilizing and iron-chelating bacterium, mobilizing phosphorus, iron, and key micronutrients via secretion of organic acids and siderophores, especially valuable in low-fertility and alkaline soils.
Stimulates Root Growth and Early Vigor - As a biostimulant and plant health bacteria, P. protegens produces auxin-like compounds and other metabolites that stimulate root elongation, seedling emergence, and nutrient uptake efficiency.
Supports Soil Health and Organic Matter Transformation - Contributes to soil structure and fertility by enhancing enzymatic breakdown of organic residues and improving humus formation. A valuable soil fertility microbe for regenerative and organic agriculture.
Compatible with Organic and Sustainable Farming Practices - Works synergistically with composts, mycorrhizae, and other microbial fertilizers for plants. Recommended for use in fertigation, seed treatment, in-furrow application, and soil drench programs across organic and sustainable systems.
Advanced Microbial Inoculants from a U.S. Manufacturer - We produce high-quality Pseudomonas protegens biofertilizers, available in commercial, bulk, and private-label formats. Whether you’re managing certified organic acreage or formulating custom microbial blends, our production facility delivers premium consistency and performance.

Looking to buy a Pseudomonas biofertilizer? Our Pseudomonas protegens is a top choice among growers seeking microbial diversity, nutrient efficiency, and soil resilience. Trusted across regenerative, conventional, and organic systems, it’s widely used in biostimulants and soil probiotic blends to improve root health, nitrogen availability, and long-term soil fertility.

Whether you're looking for agricultural bacteria for sale for large-scale application or developing a custom product with phosphate-solubilizing bacteria, Applied Biotech Industries is a trusted U.S. microbial producer offering bulk, wholesale, and private-label solutions.

Concentration (CFU/g):

10 billion (1 × 10¹¹) CFU/g dry powder
Custom concentrations available upon request

Particle Size (Mesh):

Passes through 100 mesh sieve

Packaging Options:

22 lb (10 kg) pails
44 lb (20 kg) pails
340 lb (155 kg) drums
Smaller custom packaging available on request

Shelf-life:

1 month at room temperature
1 year refrigerated
2 years in freezer

Storage Recommendations:

Store in a cool, dry location, ideally refrigerated, away from direct sunlight.
Reseal container tightly after each use.

Soil Application (Drip, Drench, or Fertigation)

Dosage:

40–100 grams per acre (100–250 grams per hectare)

Frequency:

Apply during early root establishment or transplanting
Reapply every 2–4 weeks during active growth stages as needed

Application Method:

Dissolve thoroughly in water (use enough water to reach the root zone)
Use an agitation tank or manual stirring to maintain microbial suspension
Apply through drip irrigation lines, micro-sprayers, or fertigation systems, positioning flow as close to the root zone as possible
Flush the system after application to prevent clogging or residue buildup

Do not tank mix with herbicides, fungicides, bactericides, or chemical pesticides.

In-Furrow

Dosage:

2–4 grams per acre (5–10 grams per hectare)

Frequency:

Apply once at planting to target root initiation and early growth stages.

Application Method:

Dissolve microbial powder thoroughly in water.
Apply directly into the seed furrow or planting trench at seeding time.
If using irrigation systems for delivery, ensure solution contacts the root zone.
Maintain agitation to prevent settling.

When tank-mixing with fertilizers:

Dilute fertilizer fully in water first before adding microbial powder
Do not mix with herbicides, fungicides, bactericides, or pesticides

Foliar Spray

Dosage:

40–100 grams per acre (100–250 grams per hectare)

Frequency:

Begin at vegetative or pre-flowering stages
Reapply every 7–14 days, especially during periods of high stress

Application Method:

Apply in the early morning or evening to reduce UV exposure
Use a non-ionic surfactant or wetting agent for improved adhesion
Ensure thorough coverage of foliage, including undersides of leaves
Agitate spray solution continuously during application

Seed Treatment

Dosage:

1–3 grams per kg of seed

Frequency:

Single application before planting

Application Method:

Mix thoroughly with a sticking agent (e.g., sugar solution, gum arabic)
Coat seeds evenly, ensuring full surface coverage
Allow seeds to dry gently in the shade before sowing

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Safety Data Sheet (SDS)

Tech Sheet

1. Siderophore Production and Iron Mobilization

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Key Findings:

P. protegens Pf-5 produces its own siderophore, pyoverdine, even in the presence of siderophores from other strains.
This strategy provides a competitive advantage by limiting iron availability to competing microbes.

2. Root Colonization and Plant Growth Promotion

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Key Findings:

P. protegens MP12 effectively colonizes grapevine roots and shoots.
Demonstrates potential as a plant growth-promoting bacterium through successful endophytic and rhizospheric colonization.

3. Induced Systemic Resistance by Fluorescent Pseudomonas spp.

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Key Findings:

Fluorescent Pseudomonas species, including P. protegens, can trigger ISR in plants.
ISR activation involves jasmonic acid and ethylene signaling pathways, enhancing plant defense mechanisms.

4. Adaptation to Rhizosphere Microbiomes

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‍Key Findings:

P. protegens CHA0 adapts its colonization strategy in response to different soil microbial communities.
Demonstrates flexibility and resilience in diverse rhizosphere environments.

5. Auxin and Ammonia Production by P. protegens MP12 for Root Development

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Key Findings:

P. protegens MP12 produces siderophores and ammonia, contributing to enhanced plant growth.
The strain exhibits plant growth-promoting traits, including the production of indole-3-acetic acid (IAA), which stimulates root development.

**6. Heavy Metal Tolerance and Plant Growth Promotion by Pseudomonas protegens Isolates**

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Key Findings:

P. protegens isolates demonstrate tolerance to heavy metals and possess plant growth-promoting abilities.
The strains can solubilize inorganic phosphate and produce siderophores, enhancing nutrient availability to plants.

7. Phosphate Solubilization and Siderophore Production by Pseudomonas protegens

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Key Findings:

Pseudomonas protegens CP17 solubilizes phosphate through the production of organic acids, including thioacetic, glutamic, and propionic acids.
The strain produces siderophores and indole-3-acetic acid (IAA), contributing to enhanced nutrient availability and plant growth.

8. Contribution of Siderophores to Soil Fitness in Pseudomonas protegens

🔗 Read the Study

Key Findings:

P. protegens Pf-5 synthesizes two major siderophores: pyoverdine and enantio-pyochelin.
These siderophores enhance the bacterium's survival and competitiveness in soil environments by facilitating iron acquisition.

Pseudomonas protegens